eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2019-12-28 16 04 789 795 10.13005/bbra/2795 34660 article Mitra Mazinani 1 Abas Ali Naserian 2 Brian Rude 3 Reza Valizadeh 2 Abdolmansur Tahmasbi 2 Student of Ruminant Nutrition, Ferdowsi University of Mashhad, Mashhad, Iran Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Department of Animal and Dairy Sciences, Faculty of Agriculture, Mississippi State University, USA In this experiment, essential amino acid (Methionine) and two chemical compound, were used to make ligands that produce pH-sensitive amino acids that are stable in the rumen and absorbable in the post rumen part of the digestive tract. The treatments were made with heat and steer, reflux and ultrasound reaction at different times, temperatures and solvents to make new bonds and pH-sensitive amino acid ligands. During the first 8 hours of incubation (rumen phase) 55.42% of RPMet1 was released, during the 2 hours (abomasum phase) 91.00% of the compound had been released and during the final hours up to 35h (intestine phase) 93.21% had been released and 6.79% of this product was stable. About the RPMet2 figure shown that in first 8 hours of incubation (rumen phase) 49.25% of RPMet1 was released, in next 2 hours (abomasum phase) 87.93% of the compound was released and in the final hours up to 35h (intestine phase) 94.05% was released and 5.95% of this product was stable. Result shows that this chemical method increased retention time in rumen and the bond is reversible in lower pHs, similar to the abomasum. https://www.biotech-asia.org/vol16no4/production-of-rumen-protected-essential-amino-acids-with-chemical-technique/ Essential Amino Acids; Methionine; Rumen Protected