eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-12-22 13 4 2273 2279 10.13005/bbra/2394 17024 article Zahra Akbari Jonoush 1 Fatemeh Yazdian 2 Reza Mansori 1 Meisam Omidi 3,4 Department of Immunology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. Faculty of New Science and Technology, University of Tehran, Iran. Protein Research Centre, Shahid Beheshti University, Tehran, Iran. Medical Nano-Technology and Tissue Engineering Research Center, Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Prostate specific antigen (PSA), as a tumor biomarker, plays an important role in early cancer detection, prognosis prediction and treatment evaluation for prostate cancer patients.  In particular, therefore, identification of sensitive and specific methods for early cancer detection is required.so one of the method for early cancer detection are Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles. Herein, we develop a novel LSPR biosensor for the detection of PSA. First, gold nanoparticle was prepared using turkevich method. To achieve the goal, anti-PSA was covalently conjugated to gold Nano spheres using 11-Mercaptoundecanoic acid (MUA). Finally, sample serum was investigated by localized surface Plasmon resonances and finally, the results obtained by LSPR and Chemiluminescence assay were compared with each other. The results suggested that LSPR sensor had good potential and good sensitivity in detecting of total PSA. The proposed Nano bio-optical sensor delivered a good sensitivity with average sensitivity 1.1 ng/ml, and a low detection limit of o.9 ng/ml. Our study represents the application of the LSPR biosensor in prostate cancer, and demonstrates that this method have good sensitivity, selectivity the and short assay time https://www.biotech-asia.org/vol13no4/localized-surface-plasmon-resonance-biosensor-for-detection-of-serum-prostate-specific-antigen-in-prostate-cancer-patients/ localized surface plasmon resonance; biosensor; prostate cancer biomarker; prostate specific antigen