eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-12-22 13 4 2117 2123 10.13005/bbra/2372 16740 article Roy Amit 1 Prasad Pushpa 1 Columbia Institute of Pharmacy, Tekari, Raipur (CG)-493111, India. The diabetes can be controlled by inhibiting major carbohydrate hydrolyzing enzymes lead to reduce postprandial hyperglycemia. The aim of the study was to evaluate the antidiabetic activity of oven dried extract (ODE) and lyophilized extract (LE) of Calocybe indica using enzyme inhibitory assays. The ODE and LE were tested against α-amylase, α-glucosidase, and dipeptidyl peptidase-IV (DPP-IV). The ODE demonstrated 50% inhibitory activity for α-amylase, α-glucosidase and DPP-IV enzyme at 62.18 μg/ml, 47.77 μg/ml and 91.84 μg/ml, respectively. The LE revealed 50% inhibitory activity for α-amylase, α-glucosidase and DPP-IV enzyme at 38.11 μg/ml, 28.09 μg/ml and 60.91 μg/ml, respectively. Furthermore, the IC₅₀ values of LE are nearer to IC₅₀ values of standard drug Acarbose. The lyophilized extract possessed higher enzymatic inhibitory activity compared to oven dried extract due to restoring efficiency of secondary metabolites. https://www.biotech-asia.org/vol13no4/assessment-of-mechanism-of-action-of-antidiabetic-activity-of-calocybe-indica-by-enzyme-inhibitory-activity/ Calocybe indica; α-amylase; α-glucosidase; dipeptidyl peptidase-IV