eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-09-25 13 3 1639 1643 10.13005/bbra/2311 15796 article Mohammadreza Nazem 1 Mehran farhoodi Moghadam 2 Ghasem Akbari 1 Mohammad Amin Eslampour 1 Department of Theriogenology Veterinary Medicine, Science and Research branch, Islamic Azad University, Tehran, Iran. Department of Theriogenology Veterinary Medicine, Islamic Azad University, Karaj, Iran. Maintenance of immature oocytes before the onset of puberty help to do next manipulation. The aim of study was to use Embryo Holding with and without the use of the incubator for transfering oocytes to the laboratory and examining effects on oocytes maturation.Cow ovaries were collected from slaughterhouse animaland 6 groups (EHT) of them were replaced on the Embryo Holding (Syngro^@) and kept in darkness in a styrofoam container with thermo packs at 4°C, 22°C, 38.5°C for 12, 24 hours ( EHT-12h, EHT-24h ).One group (C₁) was replaced on the standard medium IVM.After staining , the highest percentage of oocytes maturation was 92/2 % in the C₁ while it had significantly different from all groups EHT (P≤0/05). In the EHT-12h numerically had the highest percentage of maturation in 22°C (80%) and they didn’t have significant difference with groups EHT-12h (P>0/05). In EHT-24h, the highest percentage of oocytes maturation were in 38.5°C (54.3%) and they had statistically significant difference with 4°C (P≤0/05). Embryo Holding media in our study showed that can be helpful for maintenance of immature oocytes until 12 hours at room temperature and without incubation. https://www.biotech-asia.org/vol13no3/effect-of-embryo-holding-on-bovine-oocyte-maturation-outside-the-incubator/ Embryo Holding; Bovine; Oocyte; Maturation; Incubator