eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-09-25 13 3 1291 1302 10.13005/bbra/2269 15932 article Anggi Shestita Wulandari 1 Olivia Mayasari Tandrasasmita 1 Raymond Rubianto Tjandrawinata 1 Dexa Laboratories of Biomolecular Sciences, PT Dexa Medica, Industri Selatan V, Block PP No. 7, Jababeka Industrial Estate II, Cikarang, West Java, 17550, Indonesia. Lactobacillus fermentum DLBSA204 is an isolated lactic acid bacterium (LAB) from human breast milk. This study focused on the effects of L. fermentum DLBSA204 on macrophage cell proliferation with MTS assay using murine RAW 264.7 macrophage-like cell line as well as related molecular changes associated with macrophage activation. It was demonstrated that L. fermentum DLBSA204 can activate macrophage cell by increasing Nitric Oxide and cytokine expression level. Moreover, this LAB was able to increase phagocytic activity of macrophage. L. fermentum DLBSA204 was also able to inhibit the adhesion of Streptococcus pneumoniae on lung cells. In conclusion, L. fermentum DLBSA204 works as an immunomodulator and macrophage activator. https://www.biotech-asia.org/vol13no3/immunomodulatory-and-macrophage-activating-activity-of-lactobacillus-fermentum-dlbsa204-in-response-to-respiratory-infection-in-a-cellular-model/ Lactobacillus fermentum; immunomodulator; macrophage; Streptococcus pneumoniae